Confocal laser scanning microscopy is a technique using laser beam to generate the high resolution images of examined sample. The general rule in this type of microscopy is a detection of irradiated light after absorbation by the sample. The high-sensitivity 38-channel Quasar detector allows for spectral imaging, linear unmixing, and high color count (>4) image acquisition. This spectral device may be run in full spectral mode for emission fingerprinting and linear unmixing with a resolution of 8 nm, or in virtual channel mode where groups of individual channels are pooled onto virtual detectors.
TIRFM (total internal reflection fluorescence microscopy) is used for imaging in living cells. With this instrument we can track the moves of cells in physiological conditions. Specailly designed optics and cameras allow us to use cells with attached fluorophores to see how they behave. The system operates by bouncing a laser beam off of the coverslip so that photons never hits the specimen directly; however the radiating evanescent wave is sufficient to illuminate fluorescent structures nearby. Tool is perfect to examine cell membranes structure and dynamics and for resolving single molecules..