DNA Repair in Cancer Treatment
Personalised medicine is an approach to improve treatment of an individual patient by taking into account the specifics of an individual’s physiology. One aspect of this is an assessment of patient’s genomic profile. More specifically, the evaluation of DNA repair efficiency provides a unique opportunity to apply targeted cancer therapies (PARP inhibitors are the best known example). The main aim of our study is to identify other DNA repair pathways involved in cell chemosensitivity by e.g. RNAi knockdown screening in the hTERT RPE-1 cell line.
Nucleotide Excision Repair proteins inside living bacteria
Our aim is to understand the way in which DNA repair affects the physiology of an individual cell, as well as the whole organism. We hope to discover not only how DNA repair is executed and regulated but also how DNA repair contributes to other aspects of a cell’s well-being.
Our approach is to “look” at individual molecules, both in vitro and in vivo, as they find damage sites, recruit other partners participating in the repair process and perform the repair. We use smFRET assays in vitro to mechanistically understand the repair process. Furthermore, we use super-resolution Photoactivated-Localization Microscopy (PALM) to follow individual enzymes within living cells as they perform the repair process. Moreover, we analyse the repair “scars” ie. mutation signatures accumulating when a given repair pathway fails.
A short video summarising Nucleotide excision repair in E. coli.